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ژن نشانگر انتخابی
Different ap proaches have been devised for the integration of a cloned gene together with a selectable marker gene into an S. cerevisiae chromosome.
Briefly, a functional selectable marker gene and a heterologous gene equipped with yeastspecific transcription and translation control sequences are inserted between two DNA segments derived from the ends of a nonessential yeast gene.
Alternatively, some YAC vectors contain a selectable marker gene that is independent of the cloning site.
A selectable marker gene (LEU2) and a gene of interest (GOI) with transcription and translation control elements (not shown) are inserted into a yeast integrating plasmid between two segments (A1 and A2) from the ends of a nonessential yeast gene A.
The basic features include a gene of interest under the control of promoter and transcription termination sequences from the P. pastoris AOX1 gene, an E. coli origin of replication and selectable marker gene, and a yeast selectable marker gene (Fig. 3.31).
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